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Sequencing Cloning | Invitrogen TOPO PCR Cloning
Learn More
https://www.thermofisher.com/PCR Cloning/TOPO
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For sequencing get pCR4-TOPO TA vector for direct ligation of Taq-amplified PCR products. Get high-quality cloning reagents to enable reliable, consistent performance. Buy now.
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-based%2bsequence-tagged%2bsite%2b(STS)-content%2bmaps%252C%2bwhich%2bprovide%2bboth%2bcollections%2bof%2boverlapping%2bYAC%2bclones%2band%2bordered%2bsets%2bof%2bSTSs.%2bFor%2bvarious%2btechnical%2breasons%252C%2bYACs%2bare%2bnot%2bideal%2btemplates%2bfor%2bDNA%2bsequencing.%2bInstead%252C%2bthe%2bmapped%2bSTSs%2bcan%2bbe%2bused%2bto%2bisolate%2bsmaller-insert%2bbacterial%2bartificial%2bchromosome%2b(BAC)%2b(or%2bP1-derived%2bartificial%2bchromosome%2b%5bPAC%5d)%2bclones%252C%2bwhich%2bin%2bturn%2bcan%2bbe%2banalyzed124%252C%2b125%252C%2b272%2band%2bassembled%2binto%2bcontigs.%2bTypically%252C%2bthe%2bresulting%2bnascent%2bBAC%2bcontigs%2bare%2bsmaller%2bthan%2bthe%2bcorresponding%2bYAC%2bcontig.%2bHowever%252C%2bthe%2bgaps%2bbetween%2bBAC%2bcontigs%2bcan%2bbe%2bfilled%2bby%2bvarious%2bmethods%2b(e.g.%252C%2bby%2bdeveloping%2bnew%2bSTSs%2bfrom%2bappropriate%2bBAC%2bends%2band%2bthen%2bisolating%2badditional%2boverlapping%2bclones).%2bFrom%2bthe%2bresulting%2bBAC%2bcontigs%252C%2bminimally%2boverlapping%2bBACs%2b(shown%2bas%2bdotted%2blines)%2bcan%2bbe%2bselected%2band%2bindividually%2bsubjected%2bto%2bshotgun%2bsequencing%2b(see%2bthe%2btext%2band%2bFig%2b)..jpg&cdnurl=https%3a%2f%2fth.bing.com%2fth%2fid%2fR.0be35b5efdea6bb4f50c0a62eaf0e1c8%3frik%3dlrtNv1lhQDKMFg%26pid%3dImgRaw%26r%3d0&exph=768&expw=1024&q=Clone+Contig+Sequencing+Approach&simid=608022754193860152&FORM=IRPRST&ck=9D1726D9B13EF686DB4D3B0A60817977&selectedIndex=24&itb=0)
