To cross-link by UV irradiation: (i)Place the damp membrane on a dry piece of blotting paper. (ii) Irradiate at 254 nm to cross-link the DNA to the membrane. 21| Proceed directly to hybridization ...

The Southern blot is the most common way to analyse restriction endonuclease ... then transferred to the membrane by capillary transfer or electrophoretic techniques. This immobilizes the DNA ...

Results: 111 of the 206 sera were positive for HBV DNA by dot blot hybridization. 78% of these patients had HBeAg and 7.7% anti-HBc. In 60 (92.3%) of the anti-HBc positive sera no HBV DNA could be ...