After hybridization, bind the sample to streptavidin-coated magnetic beads and wash; recover the selected DNA as described for the primary selection (Steps 11–15). It is very important to use ...
This immobilizes the DNA fragments, allowing specific sequences to be identified by hybridization with single-stranded probes. Before capillary transfer, several steps must be taken to prepare the DNA ...
For the cell biologist, identifying changes in gene expression using DNA microarrays is just the ... reverse transcription is a helpful follow-up step that allows expression of selected genes ...
The protocol process with two different deck configurations is extended over two days due to a 16-hour hybridization stage. Step 1: Digest genomic DNA with restriction enzymes. Normalized genomic ...