Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the measurement of absolute signal levels, independent of sensor concentration.
Fluorescence resonance energy transfer (FRET ... The conventional method for determining FRET is to compare the donor intensity of the donor–acceptor sample to that of the donor only sample ...
and a corresponding fluorescence intensity histogram is displayed for further analysis. The LUNA-FL™ Dual Fluorescence Cell Counter is integrated with on-board flow cytometry type gating software.