A scaleable method for the liquid-phase separation of plasmid DNA from RNA. In the course of experiments on the potential of nucleic acid compaction for enhancement of chromatographic adsorption ...

This diagram illustrates the creation of recombinant DNA using a plasmid as a vector. The host plasmid is cleaved by restriction endonucleases, creating sticky ends. These sticky ends allow for the ...

Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called ...

Recombinant DNA is one of the first stories in modern biotechnology history. The National Museum of American History contains collections which trace this foundational technique of genetic engineering ...

In the context of the NIH Guidelines for Research Involving Recombinant DNA Molecules, recombinant DNA molecules are defined as either: molecules that are constructed outside living cells by joining ...

These Biosafety–Recombinant and Synthetic DNA policies and procedures exist to ensure that all research and activities involving the use of altered or synthetic and potentially hazardous DNA or RNA ...

Also, prime-boost immunization using epidermal delivery of 4 µg DNA by needleless device followed by a low dose of MVA may be more immunogenic than intramuscular delivery of 1 mg DNA followed by ...

Genetic engineering, also known as genetic modification, is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to ...

Recombinant yeast cells can be used as a vector to produce proteins that would normally be found in animals or plants. Recombinant yeast cells are used during DNA Recombinant technology as they ...

Here we show that a heterologous prime-boost vaccination regime of DNA either intramuscularly or epidermally, followed by intradermal recombinant modified vaccinia virus Ankara (MVA), induces high ...