Loop-mediated isothermal amplification - Wikipedia
Schema of a LAMP of nucleic acid biomarkers from raw untreated wastewater samples, to rapidly quantify human-specific mitochondrial DNA (mtDNA). [6] The amplification product can be detected via photometry, measuring the turbidity caused by magnesium pyrophosphate precipitate in solution as a byproduct of amplification. [7] .
Detecting amplicons of loop‐mediated isothermal amplification
2019年8月13日 · Loop‐mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real‐time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA.
Real-time turbidimetry of LAMP reaction for quantifying template DNA
2004年5月31日 · In this study, we designed an apparatus (real-time turbidimeter) capable of maintaining the LAMP reaction solutions in commercially available 0.2 ml PCR tubes at the optimum temperature (60–65 °C) and continuously measuring the turbidity of …
Reduced False Positives and Improved Reporting of Loop …
2019年5月14日 · Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as an alternative to PCR based methods. There are numerous reported techniques...
Detection of Loop-Mediated Isothermal Amplification Reaction …
2001年11月23日 · Since an increase in the turbidity of the reaction mixture according to the production of precipitate correlates with the amount of DNA synthesized, real-time monitoring of the LAMP reaction was achieved by real-time measurement of turbidity.
Recent advances in loop-mediated isothermal amplification (LAMP…
2022年1月1日 · Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on …
Principles and Applications of Loop-Mediated Isothermal ... - MDPI
2022年10月10日 · Wang et al. developed a lab-on-chip device for the rapid, automatic, and sensitive detection of viable Salmonella typhimurium using LAMP and smartphone-based real-time turbidity monitoring . The viable bacterial DNA was amplified, and the turbidity of the LAMP product was monitored in real time to quantitatively detect bacteria.
LAMP real-time turbidity detection for fowl adenovirus
2019年7月23日 · In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established. The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/μL of extracted DNA.
Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP ...
2018年4月3日 · This new real-time LAMP technology, henceforth, referred to as Fluorescence of Loop Primer Upon Self Dequenching-LAMP (FLOS-LAMP), was optimized to detect a clinically important human...
Loop-mediated isothermal amplification (LAMP): principle
2015年1月4日 · Loop-mediated isothermal amplification (LAMP), a newly developed gene amplification method, combines rapidity, simplicity, and high specificity. Several tests have been developed based on this method, and simplicity is maintained throughout all steps, from extraction of nucleic acids to detection of amplification.