Embryo-scale reverse genetics at single-cell resolution | Nature
2023年11月15日 · Here we present the ‘zebrafish single-cell atlas of perturbed embryos’: single-cell transcriptomic data from 1,812 individually resolved developing zebrafish embryos, encompassing...
Single-cell atlas of mutant zebrafish embryos | Nature Genetics
2023年12月18日 · The study presents the ‘zebrafish single-cell atlas of perturbed embryos’ dataset and a high-resolution phenotyping approach for whole organisms, and sheds light on the effect of...
A multimodal zebrafish developmental atlas reveals the ... - Cell …
2024年10月24日 · In this study, we introduce Zebrahub, a dynamic atlas of zebrafish embryonic development that integrates single-cell sequencing time course data with lineage reconstructions facilitated by light-sheet microscopy.
Zebrahub
Zebrahub is a comprehensive atlas of zebrafish embryonic development that combines single-cell RNA sequencing and live light-sheet imaging. Its aim is to provide a complete cartography of cellular lineages in space, time, and molecular domains, an essential step toward understanding how organisms develop.
A single-cell transcriptome atlas for zebrafish development
2020年3月15日 · Our single-cell transcriptome atlas encompasses transcriptional profiles from 44,102 cells across four days of development using duplicate experiments that confirmed high reproducibility. We annotated 220 identified clusters and highlighted several strategies for interrogating changes in gene expression associated with the development of ...
A single-cell transcriptome atlas for zebrafish development
2020年3月15日 · We have drafted an atlas from whole zebrafish embryos and larvae where single-cell transcriptomic data are compiled from samples that span four days of development (1–5 days post fertilization). Zebrafish is an excellent model system for producing an atlas of vertebrate gene expression over organogenesis.
Spatio-temporal mRNA tracking in the early zebrafish embryo
2021年6月7日 · Furthermore, we establish a method for high-throughput single-cell RNA labeling in early zebrafish embryos, which enables us to follow the fate of individual maternal transcripts until...
Single-cell transcriptome analysis of the zebrafish embryonic …
2021年7月7日 · Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis.
Single-cell mapping of gene expression landscapes and lineage in …
2018年4月26日 · Here we applied single-cell RNA sequencing to >92,000 cells from zebrafish embryos during the first day of development. Using a graph-based approach, we mapped a cell-state landscape that describes axis patterning, germ layer formation, and organogenesis.
Maximizing single cell dissociation protocol for individual zebrafish ...
2024年12月1日 · Here, we present an optimized protocol for isolating single cells from individual zebrafish embryos, offering a valuable resource for researchers interested in working with limited starting material. The protocol facilitates unique investigations utilizing individual embryos, such as inter-individual genetic differences and embryo-specific ...
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