Exosome uptake through clathrin-mediated endocytosis and ...
2014年8月8日 · In this report, utilizing selective inhibitors, molecular tools, and specific endocytosis markers, the cellular uptake of PC12 cell-derived exosomes was imaged by high-throughput microscopy and statistically analyzed. It was found that the uptake was through clathrin-mediated endocytosis and macropinocytosis.
Cellular uptake of extracellular vesicles is mediated by clathrin ...
2017年11月28日 · Recent evidence has established that extracellular vesicles (EVs), including exosomes and microvesicles, form an endogenous transport system through which biomolecules, including proteins and RNA, are exchanged between cells. This endows EVs with immense potential for drug delivery and regenerative medicine applications.
Endocytosis blocks the vesicular secretion of exosome marker
2024年5月8日 · Exosomes are secreted vesicles of ~30 to 150 nm diameter that play important roles in human health and disease. To better understand how cells release these vesicles, we examined the biogenesis of the most highly enriched human exosome marker proteins, the exosomal tetraspanins CD81, CD9, and CD63.
Exosomes as mediators of intercellular crosstalk in metabolism
2021年9月7日 · Exosomes are intercellular and intraorgan communication components with similarities to a classical endocrine system. In this review, Isaac and Reis et al. discuss basic aspects of exosome biology and the role of exosomes in metabolic homeostasis including their potential use as therapeutic agents.
The ins-and-outs of exosome biogenesis, secretion, and …
2024年2月1日 · During canonical multivesicular body (MVB) biogenesis, wave-like, repetitive, and coordinated recruitment of clathrin and endosomal sorting complexes required for transport (ESCRT) enable the production of a single intraluminal vesicle (ILV) at a time.
Background: Exosomes can transfer information between cells and facilitate tumor development. Results: PC12 cell-derived exosomes enter into BMSCs through clathrin-mediated endocytosis and macropinocytosis, and decrease the expression of TGF RII and TPM1 through miR-21.
The exosome journey: from biogenesis to uptake and intracellular ...
2021年4月23日 · Exosomes can be internalised by a clathrin-mediated endocytosis, b lipid-raft mediated, c caveolin-mediated endocytosis, d phagocytosis or e micropinocytosis. These pathways are not always mutually exclusive and can co-exist for the internalisation of a …
Mechanism of recipient cell-dependent differences in exosome …
2018年1月6日 · Recipient cells were treated with Pitstop 2, an inhibitor of clathrin-dependent endocytosis, or genistein, an inhibitor of caveolae-dependent endocytosis, and then incubated with DiO-labeled exosomes. Among the three methods examined, ultracentrifugation was the most efficient and reproducible.
Exosome Uptake through Clathrin-mediated Endocytosis and ...
In this report, utilizing selective inhibitors, molecular tools, and specific endocytosis markers, the cellular uptake of PC12 cell-derived exosomes was imaged by high-throughput microscopy and statistically analyzed. It was found that the uptake was through clathrin-mediated endocytosis and macropinocytosis.
Exosome Mediated Cytosolic Cisplatin Delivery Through Clathrin ...
Exosomes deliver cisplatin into cisplatin-resistant cancer cells clathrin-independent endocytosis and enhance the anti-cancer effect through avoiding endosome trapping. Cisplatin could be delivered by exosome through clathrin-independent endocytosis, and could evade the endosome trapping, diffused in the cytosol evenly.