Blastocyst-like structures generated from human pluripotent stem cells
2021年3月17日 · Human blastoids are amenable to embryonic and extra-embryonic stem cell derivation and can further develop into peri-implantation embryo-like structures in vitro. Using chemical perturbations,...
Blastocyst-like structures generated solely from stem cells
Here we report that trophoblast and embryonic stem cells cooperate in vitro to form structures that morphologically and transcriptionally resemble embryonic day 3.5 blastocysts, termed blastoids. Like blastocysts, blastoids form from inductive signals that originate from the inner embryonic cells and drive the development of the outer ...
Human blastoids model blastocyst development and implantation
2021年12月2日 · Here we show that naive human pluripotent stem cells cultured in PXGL medium 2 and triply inhibited for the Hippo, TGF-β and ERK pathways efficiently (with more than 70% efficiency) form...
Blastocyst-like structures generated solely from stem cells
2018年5月2日 · Here we report that trophoblast and embryonic stem cells cooperate in vitro to form structures that morphologically and transcriptionally resemble embryonic day 3.5 blastocysts, termed...
Early human embryonic development: Blastocyst formation to ... - Cell …
2022年1月24日 · The advent of improved culture conditions to maintain blastocysts in vitro for an extended period and the emerging stem-cell-based models of the blastocyst and peri-implantation embryos have provided new information that is relevant to early human embryogenesis.
Naive stem cell blastocyst model captures human embryo …
Reliable stem cell models of the early embryo would open new approaches to studying human development and infertility. Yanagida and colleagues used naive stem cells to generate human blastocyst-like structures comprising the three founding tissue layers for the extraembryonic membranes and the embryo.
Generation of human blastocyst-like structures from pluripotent stem cells
2021年9月7日 · Here we developed a three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts.
Building Blastocysts from Stem Cells - Cell Press
describe a novel way to generate blastocyst-like structures only from pluripotent stem cells. These structures mimic several aspects of the early embryo, offering a new promising tool to study this stage.
Stem Cells and the Future of Regenerative Medicine.
Embryonic stem cells (ESCs) are found in the inner cell mass of the human blastocyst, an early stage of the developing embryo lasting from the 4th to 7th day after fertilization. In normal embryonic development, they disappear after the 7th day, and begin to …
Building Blastocysts from Stem Cells - ScienceDirect
2019年9月10日 · In this issue of Stem Cell Reports, Kime et al. (2019) present a novel system to generate blastocyst-like structures starting from just one type of cells—mouse PSCs (Figure 1). They demonstrate how these constructs (termed iBLCs) morphologically resemble blastocysts (BCs), including cavity formation and the separation to outer TE-like cells ...