In-cell NMR spectroscopy: A tool to study cellular structure …
2022年1月1日 · In-cell NMR spectroscopy has emerged as a powerful tool to study the structural features of biomolecules within the cell. It has allowed the characterization of the protein structure, interactions, dynamics, and functions at atomic resolution in the cellular context.
In-cell NMR: recent progresses and future challenges
2023年6月20日 · We show how the combination of the atomic-level characterization of NMR with its application to a cellular context can provide crucial insights on cellular processes and drug efficacy with unprecedented level of detail.
In-Cell Structural Biology by NMR: The Benefits of the Atomic Scale
2022年3月31日 · In the era of large-scale screenings and deep learning, both accurate and qualitative experimental evidence are as essential as ever to understand the interior life of cells. In-cell structural biology by NMR spectroscopy can generate such a knowledge, and it does so at the atomic scale.
In-cell NMR: Why and how? - ScienceDirect
2022年10月1日 · NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as early as 1950. We have attempted to gather here in a didactic…
In-Cell NMR Spectroscopy - PMC - National Center for …
With the advent of in-cell Nuclear Magnetic Resonance (NMR) spectroscopy , these processes can now be studied within a cellular environment. In-cell NMR spectroscopy provides atomic level resolution of molecular structures under physiological conditions.
An Introduction to Biological NMR Spectroscopy* - Molecular & Cellular …
2013年4月22日 · NMR spectroscopy is a powerful tool for biologists interested in the structure, dynamics, and interactions of biological macromolecules. This review aims at presenting in an accessible manner the requirements and limitations of this technique.
Towards understanding cellular structure biology: In-cell NMR
2017年5月1日 · The changes in the chemical shifts of these nuclei results in conformational changes of the macromolecules. Thus, in-cell NMR spectroscopy will help to understand the structural foundation of biological processes in living cells.
In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology
2019年12月9日 · This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.
Cellular solid-state nuclear magnetic resonance spectroscopy
2012年2月13日 · Our results demonstrate that cellular ssNMR spectroscopy can be used to probe atomic details of integral membrane proteins and endogenous membrane-associated molecular components in a bacterial cellular setting.
In-Cell NMR Spectroscopy - Chemistry Europe
2005年9月1日 · The investigation and characterization of macromolecules in living cells by NMR spectroscopy has to overcome three main difficulties. First, the NMR signals of the molecule of interest must be distinguished from the NMR resonances of all other cellular components.