Chicken embryonic stem cells as a non‐mammalian embryonic …
2010年1月11日 · In contrast to the chicken embryonic fibroblasts (CEFs), which are entering into senescence after 10–15 passages (roughly corresponding to 20 cell generation), chicken embryonic stem cells (cESCs) continue to proliferate actively in the absence of any exogenous transforming agent and after a few passages become established and maintain a more ...
Study on immortal conditions of chicken embryonic stem cells
In this study, we showed that the chicken ESCs clones with a clear border can express alkaline phosphatase and marker proteins such as SSEA-1, SOX2, and OCT4 stably. In addition, culture medium containing 10 μmol/L of vitamin C (VC) could …
Spontaneous immortalization of chicken fibroblasts generates …
2022年12月22日 · We show that lecithin activates peroxisome proliferator-activated receptor gamma (PPARγ), inducing adipogenesis in immortalized fibroblasts. Blending cultured adipocyte-like cells with extruded soy...
Isolation of chicken embryonic stem cell and preparation of chicken …
2012年11月29日 · Chicken embryonic stem cells (ESCs) were separated from blastoderms at stage-X and cultured in vitro. Alkaline phosphatase activity and stage-specific embryonic antigen-1 staining was conducted to detect ESCs. Then, chicken ESCs were transfected with linearized plasmid pEGFP-N1 in order to produce chimeric chicken.
Chicken pluripotent stem cell lines as a convenient platform …
Pluripotent cell lines derived by reprogramming of chicken fibroblasts are called induced pluripotent stem cells (iPSCs). The avian-derived cell culture system contains enhanced genome ...
Chicken embryonic stem cells and primordial germ cells display ...
2016年2月10日 · In embryonic stem cells (ESCs) derived from blastocysts, factors involved in deposition of epigenetic marks regulate properties related to self-renewal and pluripotency. In the germ lineage, changes in histone PTMs and DNA demethylation occur during formation of the primordial germ cells (PGCs) to reset the epigenome of the future gametes.
Chicken embryonic stem cells and primordial germ cells display ...
2016年2月10日 · In embryonic stem cells (ESCs) derived from blastocysts, factors involved in deposition of epigenetic marks regulate properties related to self-renewal and pluripotency. In the germ lineage, changes in histone PTMs and DNA demethylation occur during formation of the primordial germ cells (PGCs) to reset the epigenome of the future gametes.
The evolution of chicken stem cell culture methods
2017年2月17日 · Development of a suspension cell culture process for chicken embryonic stem cells (cESCs), chicken primordial germ cells (PGCs) and chicken induced pluripotent stem cells (ciPSCs) will be an important advance for increasing the growth kinetics of these cells.
Chicken embryonic stem cells and primordial germ cells display ...
2016年2月10日 · Chicken ESCs and PGCs differ from their mammalian counterparts with respect to H3K27 methylation. High enrichment of H3K27me3 at PCH is specific to pluripotent cells in chicken. Our results demonstrate that the dynamics in chromatin constitution described during mouse development is not universal to …
RETRACTED ARTICLE: Production of viable chicken by allogeneic ... - Nature
2021年5月20日 · Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable...