Generation of transgenic chickens by the non-viral, cell-based …
In this review, we analyzed the effectiveness of important elements of the non-viral, cell-based strategy of transgenic chicken production from the literature cited in the Web of Science database, and the experiments that have been carried out in our laboratory.
Designing A Transgenic Chicken: Applying New Approaches …
Chicken possess a strong potential for recombinant protein production and can be used as a powerful bioreactor to produce pharmaceutical and nutritional proteins. Several transgenic chickens have been generated during the last two decades via viral and non-viral transfection.
US government approves transgenic chicken | Nature
2015年12月9日 · The US Food and Drug Administration (FDA) has approved a chicken that has been genetically engineered to produce a drug in its eggs.
Transgenic chickens - PubMed
Recently, several new methods have been developed that will enable the successful establishment of transgenic chickens. Retroviral vectors are used in many cases because of their ability to incorporate transgenes into host cell chromosomes in a highly efficient manner.
TRANSGENIC CHICKEN: METHODS AND APPLICATIONS
2012年8月1日 · The production of transgenic chicken has several advantages including shorter generation interval, lower cost and fecundity.
Transgenesis and genome editing in chickens - ScienceDirect
2020年1月1日 · After the successful establishment of transgenic mammals, it became clear that cultivation of chicken embryos in compromised eggshells to hatch is a necessary step in order to generate heritable transgenic chickens.
Germ cells and transgenesis in chickens - ScienceDirect
2009年3月1日 · We have developed embryo-mediated and testis-mediated transgenic systems using chicken primordial germ cells (PGCs) from embryos and testicular cells from adult males. These methods are efficient and involve minimal technical effort.
Transgenic chickens - Nishijima - 2013 - Wiley Online Library
The transgenic chicken is one candidate for a novel production process that incorporates a commercially superior “living bioreactor”. Such a method is required because a number of monoclonal antibodies have been widely used for therapy in humans.
Heavy chain-only antibodies with a stabilized human VH in transgenic …
2024年11月28日 · Transgenic bird evaluations for B-cell development and subsequent antibody discovery. Total circulating IgM or IgY titer (a) of different versions of the transgenes (VHH3/IgL-/- or VHH/tLCi) was compared to wild-type (WT) chicken based on plasma ELISA. Transgenic chickens exhibited only IgM antibodies, with no IgY isotype switching/expression.
Harnessing Gene Conversion in Chicken B Cells to Create a …
2013年11月21日 · To date, all of the transgenic animals producing human antibodies have been mammalian species, but a non-mammalian host such as chicken would access a much wider set of epitopes, since chickens have not shared a common …