Fluorescence polarization immunoassay - Wikipedia
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay, that consists of a simple prepare and read method, without the …
Fluorescence Polarization Immunoassay - an overview
Fluorescence polarization (FP) immunoassay is based on the competition between mycotoxin and a mycotoxin–fluorescein tracer for a mycotoxin-specific antibody. When a fluorophore in solution is exposed to plane-polarized light at its excitation wavelength, the resulting emission is …
Fluorescence polarization immunoassay: detection of antibody …
Fluorescence polarization immunoassay (FPA) is a homogeneous immunoassay useful for rapid and accurate detection of antibody or antigen. The principle of the assay is that a fluorescent dye (attached to an antigen or an antibody fragment) can be excited by plane-polarized light at the appropriate wa …
Fluorescence Polarization-Based Bioassays: New Horizons
For decades, these possibilities were successfully applied in fluorescence polarization immunoassays based on differences in the polarization of fluorophore emissions excited by plane-polarized light, whether in a free state or as part of an immune complex.
Immunoassays: Analytical and Clinical Performance, Challenges, …
Immunoassays (IAs) with fluorescence-based detection are already well-established commercialized biosensing methods, such as enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA).
Fluorescence Polarization (FP)—Note 1.4 - Thermo Fisher Scientific
Fluorescence polarization measurements provide information on molecular orientation and mobility and processes that modulate them, including receptor–ligand interactions, protein–DNA interactions, proteolysis, membrane fluidity and muscle contraction (Figure 1).
Fluorescence Polarization Immunoassay - an overview
Fluorescence Polarization Immunoassay (FPIA) is a method that measures the degree of polarization of fluorescent light emitted by a labeled molecule, which increases when the molecule binds to a larger molecule such as an antibody.
Fluorescence Polarization Immunoassay Theory and Application
Since then, the principles and practice of fluorescence polarization and fluorescence polarization immunoassay have been described in a number of review articles (Dandliker et al., 1964; Parker, 1973; Dandliker and de Saussure, 1970).
Fluorescence polarization immunoassays and related methods …
2008年2月10日 · Fluorescence polarization immunoassay (FPIA) is a homogeneous (without separation) competitive immunoassay method based on the increase in fluorescence polarization (FP) of fluorescent-labeled small antigens when bound by specific antibody.
Fluorescence Polarization - an overview | ScienceDirect Topics
Fluorescence polarization immunoassay (FPIA) is a homogeneous detection technique using fluorescence polarization. The structure and principle are shown in Fig. 10 (Ueda and Dong, 2014). It is a method for measuring the molecular tumbling rates of fluorescence-labeled molecules due to Brownian motion.