1.14: Acid-Fast Stain - Biology LibreTexts
The primary stain used in acid fast staining, carbol fuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat in the form of heat (steam). Steam helps to loosen up the waxy layer and promotes entry of the primary stain inside the cell.
Fuchsine - Wikipedia
As well as dying textiles, fuchsine is used to stain bacteria and sometimes as a disinfectant. In the literature of biological stains the name of this dye is frequently misspelled, with omission of the terminal -e, which indicates an amine. [3]
A protocol for combining fluorescent proteins with histological stains ...
2017年11月24日 · Consecutive staining with several dyes allows 3D co-visualisation of distinct cell wall modifications with fluorescent proteins – used as transcriptional reporters or protein localisation tools – deep within tissues. Moreover, the protocol is easily applied on hand sections of different organs.
Acid fuchsin - Wikipedia
Acid fuchsin or fuchsine acid, (also called Acid Violet 19 [1] and C.I. 42685 [1]) is an acidic magenta dye with the chemical formula C 20 H 17 N 3 Na 2 O 9 S 3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology, [1] and is one of the dyes used in Masson's trichrome stain. [2]
Acid-Fast Stain- Principle, Procedure, Interpretation and Examples
2024年11月11日 · Neelsen in 1883 used Ziehl’s carbol-fuchsin and heat then decolorized with an acid alcohol, and counter stained with methylene blue. Thus Ziehl-Neelsen staining techniques was developed. The main aim of this staining is to differentiate bacteria into acid fast group and non-acid fast groups.
Introduction to Histology Stains | histology - University of …
Aldehyde fuchsin is a deep purple dye. It stains elastic fibers and granules of beta cells in the islets of Langerhans, cartilage matrix, and stored neurosecretory product in the hypophyseal pars nervosa, among other things. In some of the Michigan collection slides, it is the only stain and therefore only elastin is demonstrated.
There are three common acid-fast staining methods, Ziehl-Neelsen (hot), Kinyoun (cold), and Auramine-Rhodamine Fluorochrome (Truant method). The emphasis in this Atlas-Protocol project will be on the Ziehl-Neelsen and the Kinyoun methods because the slides produced by these methods can be visualized using a standard bright-field microscope.
1.9: Simple Stain - Biology LibreTexts
Basic stains include methylene blue, crystal violet, malachite green, basic fuchsin, carbolfuchsin, and safranin. Acidic stains include eosin, acid fuchsin, rose bengal, and congo red. Basic stains are attracted to negatively charged molecules in the cell including nucleic acids (DNA and RNA) and some proteins.
Histology: Stains and section interpretation - Kenhub
2022年7月7日 · The stain uses a mixture of picric acid and acid fuchsin to penetrate the tissue sample, causing collagen to become red. Surrounding muscle tissues and blood cells are stained yellow. Picric acid molecules are very small, and therefore penetrate all types of tissues.
4.2: Specialized Bacterial Staining Techniques
2021年5月24日 · Because the cell wall is so resistant to most compounds, acid-fast organisms require a special staining technique. The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat.