Applications of Gene Editing in Chickens: A New Era Is on the …
New technologies including CRISPR/Cas9 make the process of gene editing easy and highly efficient in contrast to the well-established process of homologs recombination. Although gene editing in mammals, particularly mice and pigs, is vastly advanced, gene editing in chickens is entering the golden age.
CRISPR/Cas9 gene editing in a chicken model: current …
Improvements in genome editing technology in birds using primordial germ cells (PGCs) have made the development of innovative era genome-edited avian models possible, including specific chicken bioreactors, production of knock-in/out chickens, low-allergenicity eggs, and disease-resistance models.
Genome editing in chickens - ScienceDirect
2022年12月1日 · Genome editing of cultured PGCs is key to producing genome-edited chickens. Nowadays, several researchers use improved CRISPR/Cas systems, such as a high fidelity Cas9 variant (SpCas9-HF1)-mediated genome editing [48], D10A-Cas9 nickase-mediated gene disruption [49], and base editing system [50, 51], in chicken PGCs [52], [53], [54 ...
Creating resistance to avian influenza infection through genome editing …
2023年10月10日 · Chickens genetically resistant to avian influenza could prevent future outbreaks. In chickens, influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous...
CRISPR/Cas9 gene editing in a chicken model: current ... - Springer
2020年1月10日 · Improvements in genome editing technology in birds using primordial germ cells (PGCs) have made the development of innovative era genome-edited avian models possible, including specific chicken bioreactors, production of knock-in/out chickens, low-allergenicity eggs, and disease-resistance models.
CRISPR/Cas9 Targets Chicken Embryonic Somatic Cells
2016年10月3日 · Here, we show that the CRISPR/Cas9 system can precisely edit the chicken genome. We generated HIRA, TYRP1, DICER, MBD3, EZH2 and 6 other gene knockouts in two chicken cell lines using the...
Generation of genome-edited chicken and duck lines by adenovirus ... - PNAS
Here, we generated germline mosaic founder chicken and duck lines without the PGC-mediated procedures by injecting an adenovirus containing the CRISPR-Cas9 system into avian blastoderms.
Chicken genome editing for investigating poultry pathogens
2022年10月24日 · Many “transgenic” technologies, such as viral vectors, transposons, and site-directed nucleases, have been developed over the past 30 years to generate genetically modified chicken lines. By lines, we mean chicken containing an introduced DNA construct that will be heritably transferred from parent to offspring.
Precise gene editing of chicken Na+/H+ exchange type 1 …
2017年12月1日 · The results suggest that CRISPR/Cas9-mediated homologous recombination with ssODNs is an effective system for genome editing in chicken cell lines. However, development of a more efficient targeted replacement system in chickens is needed.
Applications of Gene Editing in Chickens: A New Era Is on the ... - PubMed
2018年10月9日 · Herein we review the current and future prospects of gene editing and how it possibly contributes to the development of resistant chickens against infectious diseases. Keywords: CRISPR/Cas9; Diseases; Immunoglobulins; chicken; knockout; transgenic.
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