Floxing - Wikipedia
In genetic engineering, floxing refers to the insertion of a DNA sequence (which is then said to be floxed) between two LoxP sequences, creating an artificial gene cassette which can then be …
Highly reliable creation of floxed alleles by electroporating single ...
2022年2月4日 · Floxed (flanked by loxP) alleles are a crucial portion of conditional knockout mouse models. However, an efficient and reliable strategy to flox genomic regions of any …
MyoD is a 3D genome structure organizer for muscle cell identity
2022年1月11日 · Here, we analyzed 3D genome structures in muscle cells from mice lacking the muscle lineage transcription factor (TF), MyoD, versus wild-type mice.
Nr5a2 is dispensable for zygotic genome activation but ... - Science
Nr5a2 flox /flox females were crossed with ROSA Cre-ERt2 KI/+; Nr5a2 del/+ males and embryos were collected at the 4C stage and treated with tamoxifen for 24 hours.
Generation of a Matrix Gla (Mgp) floxed mouse, followed by
2020年10月29日 · We identified insertion sites in the mouse genome, generated optimized gRNAs and Cas9 expression plasmids and constructed a donor DNA vector with two …
Increased ionizing radiation sensitivity and genomic instability in …
The potential role of H2AX in suppressing genomic instability was examined by using spectral karyotyping (SKY) to examine levels and types of chromosomal aberrations in early passage …
Three-dimensional genome structures of single diploid human cells
2018年8月31日 · We reconstructed the genome structures of single diploid human cells from a lymphoblastoid cell line and from primary blood cells with high spatial resolution, locating …
Efficient Detection of Flox Mice Using In Vitro Cre Recombination
Here, we describe a rapid screening method for flox mice, using in vitro Cre recombination that can be performed using simple enzymatic reactions and enables detection of functional flox …
Highly reliable creation of floxed alleles by electroporating single ...
2022年2月4日 · Background: Floxed (flanked by loxP) alleles are a crucial portion of conditional knockout mouse models. However, an efficient and reliable strategy to flox genomic regions of …
THOC5/FMIP, an mRNA export TREX complex protein, is essential …
2010年1月5日 · Generation of THOC5/FMIP (flox/flox) and Mx-cre THOC5/FMIP (flox/flox) mice. (A) The genomic structure of the 33,523 kb THOC5/FMIP locus is depicted. THOC5/FMIP …