KO (KEGG ORTHOLOGY) Database - GenomeNet
2024年9月1日 · The KO (KEGG Orthology) database is a database of molecular functions represented in terms of functional orthologs. A functional ortholog is manually defined in the …
The impact of DNA methylation on CTCF-mediated 3D genome
2024年3月18日 · Indeed, the complete knockout (KO) of CTCF during early embryogenesis in mice is lethal, and heterozygous KO mice are predisposed to cancer 22,23. What, then, drives …
Cohesin and CTCF emerge as building blocks of 3D genome structure
2024年10月25日 · Vertebrate genomes are highly organized in three-dimensional (3D) space. Techniques for measuring genome-wide chromatin interactions (such as Hi-C) enabled the …
Linking genome structures to functions by simultaneous single
2023年6月8日 · Liu et al. developed a multi-omics sequencing approach to profile 3D genome structures and gene expression at the same time in single cells, which enables a direct linkage …
Co-opted transposons help perpetuate conserved higher-order chromosomal ...
2020年1月24日 · By examining their contribution to genome topology, we show that TEs can contribute to regulatory plasticity by inducing redundancy and potentiating genetic drift locally …
Engineering Human Stem Cell Lines with Inducible Gene Knockout using ...
2015年8月6日 · Here, we report an efficient two-step strategy to generate iKO hPSC lines by combining CRISPR/Cas9-mediated genome editing with the Flp/FRT and Cre/LoxP system. …
Cohesin: behind dynamic genome topology and gene …
2021年3月22日 · Analysis of chromatin contacts in single cells and at nucleosome resolution evidence a highly dynamic genome topology. Cohesin is key for genome dynamism because …
The role of 3D genome organization in development and cell ...
2019年6月13日 · The 3D organization of the genome is crucial for gametogenesis, embryogenesis and cell differentiation through its modulation of transcription, DNA replication …
Gene Annotation and Pathway Mapping in KEGG | SpringerLink
KO is KEGG's own manually defined ortholog groups based on pathway information, specified by KO identifiers that start with “K.” Orthologous genes can be linked to the pathway entries to …
Integrative approaches in genome structure analysis - Cell Press
2022年1月6日 · We first discuss details of genome structural organization, both at the level of local chromatin folding and at the global nuclear scale. We then highlight a selection of recent …