Ion chromatography - Wikipedia
Preparative-scale ion exchange column used for protein purification. Ion exchange chromatography can be used to separate proteins because they contain charged functional groups. The ions of interest (in this case charged proteins) are exchanged for another ions (usually H +) on a charged solid support. The solutes are most commonly in a liquid ...
Ion Exchange Chromatography: Principle, Parts, Steps, Uses
2024年5月21日 · Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to ion exchangers. The principle of separation is thus by reversible exchange of ions between the target ions present in the sample solution to the ions present on ion exchangers.
Ion-Exchange Chromatography - Chemistry LibreTexts
2024年4月16日 · A second sub-category of liquid chromatography is known as ion-exchange chromatography. This technique is used to analyze ionic substances. It is often used for inorganic anions (e.g., chloride, nitrate, and sulfate) and inorganic cations (e.g., lithium, sodium, and …
Basic Principles of Ion Chromatography - Chemistry LibreTexts
2023年8月29日 · The basic process of chromatography using ion exchange can be represented in 5 steps: eluent loading, sample injection, separation of sample, elution of analyte A, and elution of analyte B, shown and explained below. Elution is the process where the compound of interest is moved through the column.
Ion-Exchange Chromatography: Basic Principles and Application
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful resolving ability, and ease of scale-up and aut …
What is ion exchange (IEX) chromatography? IEX is a liquid chromatography technique to separate proteins that have only slight differences in their net surface charge.
Ion Exchange Chromatography - an overview - ScienceDirect
Ion-exchange chromatography allows the separation of ions and polar molecules based on their affinity to the ion exchanger. Thus, it can be used for almost any kind of charged molecule including large proteins, small nucleotides, and amino acids.
In an ion exchange column, the stationary phase is a resin on which a usually organic coating provides a charged surface. The mobile phase is a solution with which the ions to be separated are introduced into the system; aqueous acid and base solutions, and organic, non-aqueous solutions can be used as the mobile phase.
Ion-Exchange Chromatography: Basic Principles and Application
Ion-exchange chromatography (IEC) is a fractionation technique that allows for the separation of ionizable molecules on the basis of differences in their electrostatic properties.
Ion-Exchange Chromatography: Basic Principles and Application
2016年10月12日 · Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful resolving ability, and ease of scale-up and automation have led to it becoming one of the ...