Dissecting metastasis using preclinical models and methods
2023年5月3日 · Despite the prevalence of lymph node metastasis in patients, lymphatic dissemination of cancer cells is understudied in mouse models, and there is a scarcity of methods for transplanting cells...
Lymph node metastases can invade local blood vessels, exit
2018年3月23日 · To confirm that metastatic cancer cells in a lymph node have an affinity for lymph node blood vessels, we used time-lapse multiphoton intravital microscopy to measure cancer cell migration in an optical lymph node window in mice . Dendra2-expressing metastatic cancer cells are first seen in the subcapsular sinus and later invade the cortex of ...
Tumor metastasis to lymph nodes requires YAP-dependent
2019年2月8日 · By using comparative transcriptomics and metabolomics analyses of primary and LN-metastatic tumors in mice, we found that LN metastasis requires that tumor cells undergo a metabolic shift toward fatty acid oxidation (FAO).
Lymph node metastasis in cancer progression: molecular …
2023年9月27日 · Lymph nodes (LNs) are important hubs for metastatic cell arrest and growth, immune modulation, and secondary dissemination to distant sites through a series of mechanisms, and it has been...
Lymph node blood vessels provide exit routes for metastatic
2018年3月23日 · To investigate this question, we delivered carcinoma cells into the lymph nodes of mice by microinfusing the cells into afferent lymphatic vessels. We found that tumor cells rapidly infiltrated the lymph node parenchyma, invaded blood vessels, and seeded lung metastases without involvement of the thoracic duct.
Inducible mouse models of colon cancer for the analysis of ... - Nature
2020年12月14日 · Invasive growth is rarely seen when tumors are induced by AOM in wild-type mice. However, when AOM is repetitively applied to mice on a p53-deficient background in intestinal epithelial cells,...
Lymph node metastases can invade local blood vessels, exit the node …
2018年3月23日 · Lymph node metastases in cancer patients are associated with tumor aggressiveness, poorer prognoses, and the recommendation for systemic therapy. Whether cancer cells in lymph nodes can seed distant metastases has been a subject of considerable debate. We studied mice implanted with cancer cells (ma …
Cancer in Lymph Nodes May Help Tumors Metastasize - NCI
2022年5月27日 · For more than a century, researchers have debated whether this invasion of lymph nodes helps the cancer spread (or metastasize) to other organs. A new study in mice suggests that it is an important step for cancer metastasis, and details how that happens.
Management of lymph node metastasis via local chemotherapy …
2021年1月10日 · Here, we established an experimental LNM model in mice by directly seeding cancer cells into a lymph node (LN), which developed spontaneous LNM-borne distant metastasis (DM) in the absence of a primary tumor.
A model system for studying superselective radiotherapy of lymph node …
2019年5月18日 · Utilizing mice with swollen lymph nodes, we succeeded in irradiating individual metastatic lymph nodes through a hole in a lead shield. This system enabled us to increase the radiation dose to >8 Gy (the lethal dose for total-body irradiation) and evaluate both direct and abscopal antitumor effects.