Neuronal density, size and shape in the human anterior cingulate cortex ...
2004年3月15日 · We stained adjacent sections of area 24b from 16 adult brains with cresyl violet or NeuN. We measured the density of pyramidal and non-pyramidal neurons, and the size and shape of pyramidal neurons, in laminae II, III, Va, Vb …
NeuN - Wikipedia
NeuN (Fox-3, Rbfox3, or Hexaribonucleotide Binding Protein-3), a protein which is a homologue to the protein product of a sex-determining gene in Caenorhabditis elegans, is a neuronal nuclear antigen that is commonly used as a biomarker for neurons.
Neuronal nuclear antigen (NeuN): a marker of neuronal maturation in …
1998年3月1日 · The reason for failure of NeuN to recognize these fetal neurons is unknown. Purkinje cells of the cerebellar cortex are not stained in the developing [1] or mature brain [2], and the present study confirms that they also lack immunoreactivity for NeuN in the immature human brain at all gestation ages. The lack of immunoreactivity for NeuN by ...
Neuronal density, size and shape in the human anterior cingulate cortex …
2004年3月15日 · First, to provide quantitative data regarding key neuronal morphometric parameters in the anterior cingulate cortex. Second, to compare the results of conventional Nissl staining with those observed after immunostaining with NeuN, an antibody becoming widely used as a selective neuronal marker.
Distinction of Neurons, Glia and Endothelial Cells in the Cerebral ...
We describe systematically and in detail the cytological features of neurons and glial cell types in the cerebral cortex of the macaque monkey and the human using semithin and thick sections stained for Nissl. We used this classical staining technique …
Quantification of Neuronal Density across Cortical Depth Using ...
A new framework for measuring densities of immunolabeled neurons across cortical layers was implemented that combines a confocal microscopy sampling strategy with automated analysis of 3D image stacks. Its utility was demonstrated by quantifying neuronal density in macaque cortical areas V1 and V2.
Automatic detection of neurons in NeuN-stained histological …
2019年4月1日 · In this paper we propose a new method for automatic detection of neurons in histological sections of the human brain cortex, based on anisotropic diffusion. The anisotropic diffusion is modeled using a partial differential equation (PDE) and is applied to high resolution microscopy images of the brain in order to detect neurons.
Novel Insights into NeuN: from Neuronal Marker to Splicing …
2015年2月14日 · Immunostaining results using anti-NeuN (mAb-A60) revealed that NeuN is distributed in the nuclei of mature neurons in nearly all parts of the vertebrate nervous system. NeuN is highly conserved among species and is stably expressed during specific stages of …
NeuN is a soluble nuclear protein that binds to DNA in vitro. On immuno- blots, NeuN appears as three bands (46-48 KD). Its function is still unknown. Developmentally, NeuN expression occurs in association with terminal neuronal differentiation (Mullen et al., 1992). Recently, NeuN has been used to identify neurons in transplantation studies
NeuN: A useful neuronal marker for diagnostic histopathology
1996年11月1日 · After microwave antigen retrieval, almost all neuronal populations revealed strong immunoreactivity for NeuN in nuclei, perikarya, and some proximal neuronal processes, whereas more distal axon...