RNAi function, diversity, and loss in the fungal kingdom
In this review, we describe the diversity of RNAi mechanisms found in the fungi, highlighting recent work in Neurospora, S. pombe, and Cryptococcus. Finally, we consider frequent, independent losses of RNAi in diverse fungal lineages and both review and speculate on evolutionary forces that may drive the losses or result therefrom.
RNA interference pathways in filamentous fungi - PMC
RNA interference is a conserved homology-dependent post-transcriptional/transcriptional gene silencing mechanism in eukaryotes. The filamentous fungus Neurospora crassa is one of the first organisms used for RNAi studies.
Establishment of Neurospora crassa as a model organism for …
2020年11月6日 · We show that viral infection upregulates the transcription of RNAi components, and that Dicer proteins (DCL-1, DCL-2) and an Argonaute (QDE-2) participate in suppression of viral replication. Our...
RNA interference pathways in fungi: mechanisms and functions
RNA interference (RNAi) is a conserved eukaryotic gene regulatory mechanism that uses small noncoding RNAs to mediate posttranscriptional/transcriptional gene silencing. The fission yeast Schizosaccharomyces pombe and the filamentous fungus Neurospora crassa have served as important model systems fo …
RNA interference Pathways in Filamentous Fungi - PMC
2016年10月15日 · RNA interference is a conserved eukaryotic homology-dependent post-transcriptional gene silencing mechanism. The filamentous fungus Neurospora crassa is one of the first organisms used for RNAi studies.
Neurospora - ScienceDirect
2011年2月22日 · In addition, in vegetative cells, repeated sequences commonly generate aberrant RNAs that trigger silencing via an RNA interference (RNAi) mechanism called ‘quelling’. Finally, in meiosis, unpaired sequences, such as those resulting from insertions or deletions in one parent, cause temporary silencing by another RNAi-based mechanism known ...
RNAi-dependent and RNAi-independent mechanisms contribute …
2004年8月9日 · In Neurospora crassa, the RNAi machinery has been shown to be required for transgene-induced post-transcriptional gene silencing [ and references within]. Here we have cloned and sequenced genomic regions methylated in Lys9H3 in N.crassa to test the requirements for components of the RNAi pathway in this modification.
qiRNA is a new type of small interfering RNA induced by DNA …
2009年5月14日 · In the filamentous fungus Neurospora crassa, the RNA interference (RNAi) pathway is essential for both double-stranded RNA (dsRNA)-induced and transgene-induced gene silencing (quelling) 5.
RNAi Function, Diversity, and Loss in the Fungal Kingdom
In this review we describe the diversity of RNAi mechanisms found in the fungi, highlighting recent work in Neurospora, S. pombe, and Cryptococcus. Finally we consider frequent, independent losses of RNAi in diverse fungal lineages and both review and speculate on evolutionary forces that may drive the losses or result therefrom.
DNA Methylation Is Independent of RNA Interference in Neurospora
2004年6月25日 · Here we show that DNA methylation and HP1 localization occur normally in Neurospora in the absence of key elements of the RNA-silencing machinery. Neurospora has genes for three RNA-dependent RNA polymerase (RdRP), two dicer, two argonaute, and two RecQ helicase homologs (5).