Overview: DNA cloning (article) | Khan Academy
Definition, purpose, and basic steps of DNA cloning.
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Learn about DNA cloning and recombinant DNA techniques on Khan Academy.
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This kind of plasmid can be modified to include genes that are useful for cloning and manipulating other genes. A modified plasmid that is used for cloning is known as a vector. A successfully modified vector becomes a recombinant, or recombinant plasmid.
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Explore the principles of biotechnology and DNA delivery methods, including competent cells and transformation, with Khan Academy's educational resources.
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In transformation, prokaryotes take up DNA directly from their environment (either as a circular plasmid, or as a DNA fragment that can integrate into the prokaryote's genome through homologous recombination).
Classic experiments: DNA as the genetic material - Khan Academy
The work of the researchers above provided strong evidence for DNA as the genetic material. However, it still wasn't clear how such a seemingly simple molecule could encode the genetic information needed to build a complex organism.
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Or perhaps you’ve done some DNA cloning, trying to "paste" a gene into a circular DNA plasmid. Now, you want to check and see whether your PCR worked, or whether your plasmid has the right gene in it.
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Introduction to genetic engineering (video) | Khan Academy
Today, recombinant DNA technology enables precise gene manipulation, creating genetically modified organisms (GMOs) with enhanced traits. While offering benefits like robust crops and new medicines, it raises bioethical concerns, especially regarding human genome modification.
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what if the insertional inactivation happens in the 3,4? so that we can easily extract the 1and 2 cells of recombinant DNA easily