pWB980-ori 载体图谱和序列 - 纽普生物
pWB980 derived from pUB110 is an expression vector in Bacillus for its high copy number and high stability. It has a size of 3787 base pairs, which is considered manageable and does not impose significant metabolic burden on the host cells. One key feature of the pWB980 plasmid is its bacterial resistance attribute, specifically for kanamycin.
pWB980质粒-北京庄盟国际生物基因科技有限公司
bs168. 培养条件: 37度. 表达宿主: 枯草芽孢杆菌. 5'测序引物: 根据序列设计引物. 3'测序引物: 根据序列设计引物
High copy number and highly stable Escherichia coli–Bacillus …
pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
Cloning and expression of Staphylococcus simulans lysostaphin enzyme ...
2021年7月23日 · In this paper, we describe the cloning and expression of the lysostaphin enzyme gene in Bacillus subtilis WB600 host using pWB980 expression system. Plasmid pACK1 of S. simulans was extracted using the alkaline lysis method. Lysostaphin gene was isolated by PCR and cloned into pTZ57R/T-Vector, then transformed into Escherichia coli DH5α.
(PDF) High copy number and highly stable Escherichia coli-Bacillus ...
2020年1月22日 · The best shuttle plasmids pUC980-1 and pUC980-2, which showed copies more than 450 per cell and segregational stabilities up to 98%, were selected for heterologous expressions of an alkaline ...
(PDF) High copy number and highly stable Escherichia coli–Bacillus ...
2020年2月7日 · On the basis of pWB980, constructing an E. coli-B. subtilis shuttle plasmid could facilitate the transformation rate to Bacillus cells.
pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
High copy number and highly stable Escherichia coli-Bacillus ... - PubMed
2020年2月7日 · The optimum ori insertion site was the upstream region of BA3-1 in pWB980 which resulted in shuttle plasmids with higher copy numbers and higher stabilities. The novel shuttle plasmids pUC980-1 and pUC980-2 will be promising expression vectors in B. subtilis. Moreover, the ori insertion mechanism re …
Construction of high copy number and highly stable Escherichia …
2019年12月10日 · Background: pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to...
[PDF] High copy number and highly stable Escherichia coli–Bacillus ...
2020年1月22日 · The novel shuttle plasmids pUC980-1 and pUC980-2 will be promising expression vectors in B. subtilis. Moreover, the ori insertion mechanism revealed in this work could provide theoretical guidance for further studies of pWB980 and constructions of other shuttle plasmids.