C3 transferase gene therapy for continuous conditional RhoA …
2016年12月17日 · The bacterial enzyme C3-ADP ribosyltransferase (C3) selectively and irreversibly inhibits the activation of RhoA and stimulates axon outgrowth and regeneration. However, effective intracellular delivery of the C3 protein in vivo is limited by poor cell permeability and a short duration of action.
C3 transferase gene therapy for continuous conditional RhoA …
2016年12月12日 · RhoA, a small GTPase in the Rho family, is a key intracellular switch that mediates the effects of these extracellular regrowth inhibitors. The bacterial enzyme C3-ADP ribosyltransferase (C3) selectively and irreversibly inhibits the activation of RhoA and stimulates axon outgrowth and regeneration.
Active RhoA Exerts an Inhibitory Effect on the Homeostasis and ...
2022年6月14日 · Inhibition of RhoA by C3 transferase antagonized the inhibitory effects of RhoA and strongly enhanced VEGF‐induced angiogenic sprouting in control‐treated cells. In contrast, inhibition of RhoA effectors ROCK1/2 and LIMK1/2 (LIM domain kinase 1/2) did not significantly affect RhoA‐related effects, but increased angiogenic sprouting and ...
Rho GTPase Recognition by C3 Exoenzyme Based on C3-RhoA …
2015年8月8日 · C3 exoenzyme is a mono-ADP-ribosyltransferase (ART) that catalyzes transfer of an ADP-ribose moiety from NAD + to Rho GTPases. C3 has long been used to study the diverse regulatory functions of Rho GTPases. How C3 recognizes its substrate and how ADP-ribosylation proceeds are still poorly understood.
Endothelial RhoA GTPase is essential for - Nature
2019年8月12日 · Both VEGF and S1P stimulation induced RhoA activation in primary endothelial cells (HUVECs) and this induction was blocked by C3 toxin (exoenzyme C3) treatment (Fig. 1A,B). To identify...
RHOA inactivation enhances Wnt signalling and promotes
2014年11月21日 · RhoA inactivation in the murine intestine accelerates the tumorigenic process and in human colon cancer cells leads to the redistribution of β-catenin from the membrane to the...
C3 exoenzymes, novel insights into structure and action of …
2006年12月5日 · C3-like exoenzymes catalyze the transfer of the ADP-ribose moiety of NAD on a conserved asparagine residue in Rho (e.g., RhoA N41), which is located directly adjacent to the so-called switch I region (Fig. 2; Sekine et al. 1989; Maehama et al. 1994).
C3 Transferase Gene Therapy for Continuous RhoA Inhibition
In neurons, the bacterial exoenzyme C3 transferase irreversibly inactivates RhoA GTPase signaling to promote neuritogenesis and axon regeneration following an injury. Thus, we have adopted a gene therapy approach for the targeted inhibition of RhoA activity in the CNS by expressing C3 transferase.
RhoA signaling increases mitophagy and protects cardiomyocytes …
2022年6月27日 · These findings demonstrate that RhoA activation engages a unique mechanism to regulate PINK1 accumulation, induce mitophagy and protect against ischemic stress, and implicates regulation of...
C3 transferase gene therapy for continuous conditional RhoA …
2016年12月17日 · RhoA, a small GTPase in the Rho family, is a key intracellular switch that mediates the effects of these extracellular regrowth inhibitors. The bacterial enzyme C3-ADP ribosyltransferase (C3) selectively and irreversibly inhibits the activation of RhoA and stimulates axon outgrowth and regeneration.