PDK1 regulates cancer cell motility by antagonising inhibition
Jan 20, 2008 · In this study, we show that PDK1 is required for phosphorylation of myosin light chain and cell motility, both on deformable gels and in vivo. Depletion of PDK1 alters the localization of ROCK1...
The Function of Rho-Associated Kinases ROCK1 and ROCK2 in …
The interaction of ROCK1 and Rnd3 is counter-regulated by the phosphoinositide-dependent kinase-1 (PDK1), which competes directly with Rnd3 for binding to ROCK1. In the absence of PDK1, negative regulation of Rho/ROCK signaling by Rnd3 predominates, causing reduced actomyosin contractility and motility as shown for cancer cells.
Activation of the essential kinase PDK1 by phosphoinositide …
Apr 6, 2022 · We propose a model of lipid-dependent PDK1 activation in which PH domain-mediated autoinhibition is relieved by PIP 3 or PI (3,4)P 2 binding, leading to strongly cooperative kinase domain...
Competing for ROCK1 | Science Signaling - AAAS
Feb 12, 2008 · ROCK1 was not a substrate for PDK1 in vitro. In vitro experiments suggested that PDK1 and RhoE, a negative regulator of ROCK1, competed for binding to ROCK1, and lysates from cells in which PDK1 was knocked down showed enhanced binding of ROCK1 to RhoE in a pull-down assay.
ROCK-I interacts with PDK1 and phosphorylates PDK1. (A ...
Here, we examined the contribution of RhoA-associated coiled-coil containing kinases (ROCK), key players in neuritogenesis, to prion diseases. We found that overacti... ... investigate how...
Regulation of ROCK Activity in Cancer - PMC - National Center for ...
Sep 9, 2012 · Two homologous mammalian serine/threonine kinases, Rho-associated protein kinases (ROCK I and II), are key regulators of the actin cytoskeleton acting downstream of the small GTPase Rho. ROCK is associated with cancer progression, and ROCK protein expression is elevated in several types of cancer.
(PDF) PDK1 regulates cancer cell motility by ... - ResearchGate
Mar 1, 2008 · In this study, we show that PDK1 is required for phosphorylation of myosin light chain and cell motility, both on deformable gels and in vivo. Depletion of PDK1 alters the localization of ROCK1...
PDK1-mediated activation of MRCKα regulates directional cell …
Aug 4, 2014 · Here, we report that 3-phosphoinositide-dependent kinase 1 (PDK1) regulates epithelial directional migration and invasion by binding and activating myotonic dystrophy kinase–related CDC42-binding kinase α (MRCKα).
Double-Edge Sword of Sustained ROCK Activation in Prion …
Aug 4, 2015 · The inhibition of ROCK with Y-27632 or dimethylfasudil (i) rescues F-actin plasticity necessary for neuritogenesis, synapse connectivity and the integrity of axon/dendrites, and (ii) disrupts the ROCK/PDK1 complex, which lowers PDK1 activity and allows TACE to target back to the plasma membrane.
The nuts and bolts of AGC protein kinases - Nature
A recent study indicates that PDK1 may influence ROCK activation in a kinase-independent manner, by interacting with ROCK1 and preventing the binding of its inhibitor, RHOE 96. MAST .