Structural basis of actin filament assembly and aging | Nature
2022年10月26日 · In its monomeric form (G-actin), actin exhibits very weak ATPase activity (7 × 10 −6 s −1) (ref. 4) but polymerization into filaments (F-actin) triggers a conformational …
Structure and Organization of Actin Filaments
Actin filaments are particularly abundant beneath the plasma membrane, where they form a network that provides mechanical support, determines cell shape, and allows movement of the …
The Self-Assembly and Dynamic Structure of Cytoskeletal Filaments
Tubulin and actin (the subunits of microtubules and actin filaments, respectively) bind and hydrolyze nucleoside triphosphates (tubulin binds GTP and actin binds ATP). Nucleotide …
Biochemical and mechanical regulation of actin dynamics
2022年8月2日 · Polymerization of actin filaments against membranes produces force for numerous cellular processes, such as migration, morphogenesis, endocytosis, phagocytosis …
Dynamin regulates the dynamics and mechanical strength of the actin …
2020年5月25日 · GTP hydrolysis by dynamin triggers disassembly of fully assembled dynamin helices, releasing free dynamin dimers/tetramers and facilitating Arp2/3-mediated branched …
Regulation of actin filament assembly and disassembly in growth …
2023年1月1日 · Cellular actin exists either as globular actin monomers (G-actin) or as polymeric filaments termed F-actin or microfilaments (Pollard, 2016). Regardless whether it is present as …
Mechanism of actin polymerization revealed by cryo-EM …
2019年2月14日 · We used cryo-electron microscopy (cryo-EM) to reconstruct actin filaments with bound AMPPNP (β,γ-imidoadenosine 5′-triphosphate, an ATP analog, resolution 3.1 Å), ADP …
Actin, a Central Player in Cell Shape and Movement | Science
2009年11月27日 · The protein actin forms filaments that provide cells with mechanical support and driving forces for movement. Actin contributes to biological processes such as sensing …
Molecular basis for actin polymerization kinetics modulated by …
2024年2月8日 · In this study, we investigate how crowding modulates filament assembly kinetics using total internal reflection fluorescence (TIRF) microscopy imaging and pyrene …
Myosin Motors Drive Long Range Alignment of Actin Filaments
2010年2月12日 · At actin concentrations of above 20 μ m we found that movement of rhodamine-phalloidin-labeled actin filaments became locally aligned and ordered domains formed in …