CD14 controls the LPS-induced endocytosis of Toll-like Receptor 4
To identify proteins that specifically regulate the LPS-induced endocytosis of TLR4, we used a highly sensitive assay to detect endogenous TLR4 by flow cytometry.
Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry …
Here, we describe a flow cytometry-based method that we used recently to study the role of the ion channel TRPM7 in TLR4 endocytosis ( Schappe et al., 2018 ). The assay relies on …
Mechanisms of Toll-like receptor 4 endocytosis reveal a common …
Surface level of TLR4 was determined by flow cytometry. (F) iBMDMs were left untreated or treated with cycloheximide (50 μg/ml) for the indicated times. Cells were lysed and CD14 and …
Low doses of LPS exacerbate the inflammatory response and
2018年5月2日 · Our results show that the sequential stimulation of TLR3 and TLR4 leads to the exacerbation of the inflammatory response of monocytes together with the triggering of cell …
TRAM couples endocytosis of Toll-like receptor 4 to the ... - Nature
2008年2月24日 · Here we show that TLR4 activated these two signaling pathways sequentially in a process organized around endocytosis of the TLR4 complex. We propose that TLR4 first …
TLR4 signalling via Piezo1 engages and enhances the macrophage …
2021年6月10日 · Flow cytometry indicated that the frequency of Gr-1 + CD11b + neutrophils and F480 + CD11b + macrophages in the bone marrow, spleen, and blood, and the composition …
EGFR signaling augments TLR4 cell surface expression and
2019年11月8日 · Peritoneal lavage fluids were collected, and peritoneal macrophages were identified with F4/80. TLR4 intensity on the surface of peritoneal macrophage was analyzed by …
Role of TLR4 (Toll-Like Receptor 4) in N1/N2 Neutrophil …
2019年8月27日 · Flow cytometry was performed in brain, blood, and bone marrow (BM) from MCAO WT (wild type), MCAO TLR4-knockout (KO), and naive WT and TLR4-KO animals …
Toll-like receptors stimulate human neutrophil function
2003年10月1日 · Flow cytometry with commercially available antibodies to TLRs 1, 2, 4, and 9 demonstrated modest expression of these TLRs in freshly isolated human neutrophils (Figure …
Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
2018年7月20日 · Here, we describe a flow cytometry-based method that we used recently to study the role of the ion channel TRPM7 in TLR4 endocytosis (Schappe et al., 2018). The …